GSFS Faculty

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KIKAWADA Takahiro

(Visiting Professor/Division of Biosciences)

Department of Integrated Biosciences/Molecular biology of extreme tolerant organisms

Career Summary

1992: B.A. in Agriculture - Iwate University
1994: M.A. in Agriculture - Graduate School of Iwate University
1994: Research Scientist - National Institute of Serological and Entomological Sciences
2001: Research Scientist - National Institute of Agrobiological Sciences
2005: Principal Researcher - National Institute of Agrobiological Sciences
2009: Ph.D. from Tokyo Institute of Technology
2015: Associate Professor - University of Tokyo
2023: Group Leader (PI) - National Agriculture and Food Research Organization (NARO)
2023: Professor - University of Tokyo

Educational Activities

Graduate School: Applied Bioresource Sciences

Research Activities

Elucidation of molecular mechanisms underlying anhydrobiosis:
Water is an essential element of life. Even though most organisms will die when subjected to a severe depletion of water, there are some that can survive. Simple rehydration allows these dehydrated organisms to resume active life. The sleeping chironomid is the only insect with this anhydrobiosis capability, which is a state of suspended animation by desiccation. The desiccated larvae can tolerate a variety of stresses. Eventually, the larvae can be revived, even after exposure in outer space for over two years.

Literature

1) Mizutani K, Yoshida Y, Nakanishi E, Miyata Y, Tokumoto S, Fuse H, Gusev O, Kikuta S, Kikawada T. (2024) A sodium-dependent trehalose transporter contributes to anhydrobiosis in insect cell line, Pv11. Proc Natl Acad Sci U S A. 121(14):e2317254121. doi: 10.1073/pnas.2317254121.
2) Yoshida Y, Shaikhutdinov N, Kozlova O, Itoh M, Tagami M, Murata M, Nishiyori-Sueki H, Kojima-Ishiyama M, Noma S, Cherkasov A, Gazizova G, Nasibullina A, Deviatiiarov R, Shagimardanova E, Ryabova A, Yamaguchi K, Bino T, Shigenobu S, Tokumoto S, Miyata Y, Cornette R, Yamada TG, Funahashi A, Tomita M, Gusev O, Kikawada T. (2022) High quality genome assembly of the anhydrobiotic midge provides insights on a single chromosome-based emergence of extreme desiccation tolerance. NAR Genom Bioinform. 4(2):lqac029. doi: 10.1093/nargab/lqac029.
3) Ryabova A, Cornette R, Cherkasov A, Watanabe M, Okuda T, Shagimardanova E, Kikawada T, Gusev O. (2020) Combined metabolome and transcriptome analysis reveals key components of complete desiccation tolerance in an anhydrobiotic insect. Proc Natl Acad Sci U S A. 117(32):19209-19220. doi: 10.1073/pnas.2003650117.
4) Gusev O, Suetsugu Y, Cornette R, Kawashima T, Logacheva M.D, Kondrashov A.S, Penin A.A, Hatanaka R, Kikuta S, Shimura S, Kanamori H, Katayose Y, Matsumoto T, Shagimardanova E, Alexeev D, Govorun V, Wisecaver J, Mikheyev A, Koyanagi R, Fujie M, Nishiyama T, Shigenobu S, Shibata T.F, Golygina V, Hasebe M, Okuda T, Satoh N, Kikawada T (2014) Comparative genome sequencing reveals genomic signature of extreme desiccation tolerance in the anhydrobiotic midge. Nat Commun. 5:4784. doi: 10.1038/ncomms5784
5) Kikawada T, Saito A., Kanamori Y., Nakahara Y., Iwata K., Tanaka D., Watanabe M. and Okuda T. (2007) Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells., Proc Natl Acad Sci U S A. 104(28):11585-90. doi: 10.1073/pnas.0702538104

Other publications: ORCID

Other Activities

Japanese Society for Cryobiology and Cryotechnology (Council Member: 2012-present; Associate Editor: 2010-present)
The Japanese Society for Extremophiles (Symposium committee: 2010-2013; Executive Board Member: from 2016)
Japan Transporter Research Association (Board Member: 2006-present)
The Molecular Biology Society of Japan
International Symposium-New Frontiers in Anhydrobiosis (Scientific Committee: 2014)

Future Plan

Our aim is to clarify the molecular mechanisms underlying anhydrobiosis in the sleeping chironomid.

Messages to Students

Ever tried. Ever failed. No matter. Try Again. Fail again. Fail better.
- Samuel Beckett

URL

http://www.nias.affrc.go.jp/anhydrobiosis/index_e.html