Professor Sachihiro Matsunaga of the Department of Integrated Biosciences in the Graduate School of Frontier Sciences led the research project.

Abstract

During de novo plant organ regeneration, auxin induction mediates the formation of a pluripotent cell mass called callus, which regenerates shoots upon cytokinin induction. However, molecular mechanisms underlying transdifferentiation remain unknown. Here, we showed that the loss of HDA19, a histone deacetylase (HDAC) family gene, suppresses shoot regeneration. Treatment with an HDAC inhibitor revealed that the activity of this gene is essential for shoot regeneration. Further, we identified target genes whose expression was regulated through HDA19-mediated histone deacetylation during shoot induction and found that ENHANCER OF SHOOT REGENERATION 1 and CUP-SHAPED COTYLEDON 2 play important roles in shoot apical meristem formation. Histones at the loci of these genes were hyperacetylated and markedly upregulated in hda19. Transient ESR1 or CUC2 overexpression impaired shoot regeneration, as observed in hda19. Therefore, HDA19 mediates direct histone deacetylation of CUC2 and ESR1 loci to prevent their overexpression at the early stages of shoot regeneration.

Article

Publication: PNAS Nexus（February 22, 2023）
Title: Histone deacetylation regulates de novo shoot regeneration
Authors: Haruka Temman†, Takuya Sakamoto†, Minoru Ueda, Kaoru Sugimoto, Masako Migihashi, Kazunari Yamamoto, Yayoi Inui, Hikaru Sato, Mio K. Shibuta, Norikazu Nishino, Tomoe Nakamura, Hiroaki Shimada, Yukimi Y. Taniguchi, Seiji Takeda, Mitsuhiro Aida, Takamasa Suzuki, Motoaki Seki, Sachihiro Matsunaga*
DOI: 10.1093/pnasnexus/pgad002