教育活動

先端生命科学総合演習、学融合セミナー、生命科学概論I/II、真核細胞生物学

研究活動

「植物の生きざまを分子の言葉で理解する」ためにRNAと細胞壁ポリマーといったバイオポリマーに着目し、そのダイナミクスと制御メカニズムの研究を行っています。このために、コケ、シダ、裸子植物（テーダマツ、トウヒなど）、被子植物（シロイヌナズナ、タバコ、ポプラなど）を材料に、分子遺伝学的・分子生物学的・生化学的な実験的解析や、比較ゲノム解析や比較オミクスデータ解析といったデータ解析を組み合わせて研究に取り組んでいます。
１）植物細胞の分化全能性発現を支える分子基盤の解明
２）植物の環境応答におけるバイオポリマーダイナミクスの役割の解明
３）木質バイオマス生合成の分子的理解と応用

文献

代表的原著論文（*責任著者）
1) Uy ALT, Yamamoto A, Matsuda M, Hasunuma T, Demura T, *Ohtani M (2023) The Carbon Flow Shifts from Primary to Secondary Metabolism during Xylem Vessel Cell Differentiation in Arabidopsis thaliana. Plant Cell Physiol 64: 1563–157
2) Takayanagi N, Mukai M, Sugiyama M, and *Ohtani M (2022) Transcriptional regulation of cell proliferation competence-associated Arabidopsis genes, CDKA;1, RID1, and SRD2 by phytohormones in tissue culture. Plant Biotechnol. 39, 329-333
3) Hirai R, Wang S, *Demura T, *Ohtani M (2022) Histone deacetylation controls xylem vessel cell differentiation via transcriptional regulation of a transcription repressor complex OFP1/4–MYB75–KNAT7–BLH6. Front Plant Sci 12, 825810
4) Arae T, Nakakoji M, Noguchi M, Kamon E, Sano R, *Demura T, *Ohtani M (2021) Plant secondary cell wall proteome analysis with an inducible system for xylem vessel cell differentiation. Dev Growth Differ 64, 5-15
5) Chiam NC, Fujimura T, Sano R, Akiyoshi N, Hiroyama R, Watanabe Y, Motose H, Demura T, *Ohtani M (2019) Nonsense-mediated mRNA decay deficiency affects the auxin response and shoot regeneration in Arabidopsis. Plant Cell Physiol 60: 2000-2014
6) Takenaka Y, Watanabe Y, Schuetz M, Unda F, Hill Jr JL, Phookaew P, Yoneda Y, Mansfield SD, Samuels L, *Ohtani M, *Demura T (2018) Patterned deposition of xylan and lignin is independent from that of the secondary wall cellulose of Arabidopsis xylem vessels. Plant Cell 30: 2663-2676
7) Tan TT, Endo H, Sano R, Kurata T, Yamaguchi M, *Ohtani M, *Demura T (2018) Arabidopsis VND1-VND3 contribute to xylem vessel element formation in the cotyledon. Plant Phyiol 176: 773-789
8) Bowmn JL, Kohchi T, Yamato KT., et al. (2017) Insights into land plant evolution garnered from the Marchantia polymorpha genome. Cell 171: 287-304
9) Ohtani M, Morisaki K, Sawada Y, Sano R, Uy ALT, Yamamoto A, Kurata T, Nakano Y, Suzuki S, Matsuda M, Hasunuma T, Hirai MY, *Demura T (2016) Primary metabolism during biosynthesis of secondary wall polymers of protoxylem vessel elements. Plant Physiol 172: 1612-1624
10) *Ohtani M, Takebayashi A, Hiroyama R, Xu B, Kudo T, Sakakibara H, Sugiyama M, Demura T (2015) Cell dedifferentiation and organogenesis in vitro require more snRNA than does seedling development in Arabidopsis thaliana. J Plant Res 128: 371-380
11) Xu B, *Ohtani M, Yamaguchi M, Toyooka K, Wakazaki M, Sato M, Kubo M, Hiwatashi Y, Murata T, Kurata T, Yoneda A, Kato K, Hasebe M, *Demura T (2014) Contribution of NAC Transcription Factors to Plant Adaptation to Land. Science 343: 1505-1508
12) *Ohtani M, Demura T, Sugiyama M (2013) Arabidopsis ROOT INITIATION DEFECTIVE 1, a DEAH-box RNA helicase involved in pre-mRNA splicing, is essential for plant development. Plant Cell 25: 2056-2069
13) Ohtani M, Nishikubo N, Xu B, Yamaguchi M, Mitsuda N, Goue N, Shi F, Ohme-Takagi M, *Demura T (2011) A NAC domain protein family contributing to the regulation of wood formation in poplar. Plant J 67: 499-512
14) Ohtani M, Demura T, *Sugiyama M (2010) Particular significance of SRD2-dependent snRNA accumulation in polarized pattern generation during lateral root development of Arabidopsis. Plant Cell Physiol 51: 2002-2012
15) Yamaguchi M, Goue N, Igarashi H, Ohtani M, Nakano Y, Mortimer JC, Nishikubo N, Kubo M, Katayama Y, Kakegawa K, Dupree P, *Demura T (2010)VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system. Plant Physiol 153: 906-914
16) Ohtani M, Demura T, *Sugiyama M (2008) Differential requirement for the function of SRD2, an snRNA transcription activator, in various stages of plant development. Plant Mol Biol 66: 303-314
17) Ohtani M, *Sugiyama M (2005) Involvement of SRD2-mediated activation of snRNA transcription in the control of cell proliferation competence in Arabidopsis. Plant J 43: 479-490

その他

日本植物学会、日本植物生理学会、日本植物細胞分子生物学会、日本分子生物学会、日本RNA学会、リグニン学会
Editor of "Plant Biotechnology”（2022年〜）、Editor of "Journal of Plant Research" （2021〜）Associate Editor of “Plant Molecular Biology” (2019年～）、Editorial Board of “Frontiers in Plant Science”（2018年～）等
日本学術会議連携会員

将来計画

本研究室では、「植物がどのように環境条件を捉え、応答し、細胞の増殖や分化の柔軟な制御を通して、動的な個体統御をなしえているのか？」を明らかにし、システムとしての生命機能の理解を目指します。

教員からのメッセージ

植物を材料に、生物がなぜ多様な機能を果たし得るのか、その仕組みの解明と応用に取り組んでいます。興味のある方、ぜひ一緒に、植物の謎に取り組みましょう。

ホームページのURL

https://www.ib.k.u-tokyo.ac.jp/faculty/plant_functional_analyses/